Diacetyl (Acetoin) Reductase from Aerobacter aerogenes. Kinetic Studies of the Reduction of Diacetyl to Acetoin
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چکیده
منابع مشابه
Plasmid-encoded diacetyl (acetoin) reductase in Leuconostoc pseudomesenteroides.
A plasmid-borne diacetyl (acetoin) reductase (butA) from Leuconostoc pseudomesenteroides CHCC2114 was sequenced and cloned. Nucleotide sequence analysis revealed an open reading frame encoding a protein of 257 amino acids which had high identity at the amino acid level to diacetyl (acetoin) reductases reported previously. Downstream of the butA gene of L. pseudomesenteroides, but coding in the ...
متن کاملDiacetyl and acetoin production by Lactobacillus casei.
Agitation of broth cultures of Lactobacillus casei retarded cellular dry weight accumulation but enhanced production of both diacetyl and acetoin. Addition of pyruvate overcame this retardation, but addition of sulfhydryl-protecting reagents did not. Both pyruvate and citrate enhanced accumulated dry weight of L. casei incubated without agitation, but only pyruvate increased diacetyl accumulati...
متن کاملMethod development for the determination of diacetyl and acetoin at a microwave popcorn plant.
Separate sampling and analytical methods for the determination of diacetyl and acetoin have been developed to assess workplace exposures at a popcorn processing facility have been described. Diacetyl (NMAM 2557) is efficiently recovered from an Anasorb CMS sampler tube when the composition of methanol in the desorption solvent is 1%, and acetoin (NMAM 2558) is efficiently recovered when the con...
متن کاملBiosynthesis of diacetyl in bacteria and yeast.
Both diacetyl and acetoin were produced by cell-free extracts and cultures of Pseudomonas fluorescens, Aerobacter aerogenes, Lactobacillus brevis, and Saccharomyces cerevisiae 299, whereas only acetoin was produced by cell-free extracts and cultures of Streptococcus lactis, Serratia marcescens, Escherichia coli, and S. cerevisiae strains 513 and 522. Cell-free extracts that produced diacetyl di...
متن کاملBacterial Butylene Glycol Dehydrogenase and Diacetyl Reductase* by Harold
The early literature indicates much confusion as to the origin of acetylmethylcarbinol and butylene glycol in microbiological fermentations. Happold and Spencer (1) have reviewed much of this early work and have studied in detail the conditions for the formation of acetylmethylcarbinol and butylene glycol from glucose and pyruvate by Aerobacter aerogenes. Juni (2) has demonstrated that acetoin ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1973
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1973.tb02733.x